Bestrophin 3 Antibody (OTI2H3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03253
![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]](https://resources.bio-techne.com/images/products/Bestrophin-3-Antibody-2H3-Flow-Cytometry-NBP2-03253-img0005.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]")
![Western Blot: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]](https://resources.bio-techne.com/images/products/Bestrophin-3-Antibody-2H3-Western-Blot-NBP2-03253-img0008.jpg "Western Blot: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2H3
Concentration
0.98 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
50.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Bestrophin 3 Antibody (OTI2H3)
Western Blot: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]
Western Blot: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophin 3.![Immunocytochemistry/ Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]](https://resources.bio-techne.com/images/products/Bestrophin-3-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP2-03253-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]")
Immunocytochemistry/ Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]
Immunocytochemistry/Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]](https://resources.bio-techne.com/images/products/Bestrophin-3-Antibody-2H3-Flow-Cytometry-NBP2-03253-img0006.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]")
Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253]
Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.Applications for Bestrophin 3 Antibody (OTI2H3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.98 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bestrophin 3
Alternate Names
bestrophin 3, bestrophin-3, MGC13168, MGC40411, vitelliform macular dystrophy 2-like 3, VMD2L3Vitelliform macular dystrophy 2-like protein 3
Entrez Gene IDs
144453 (Human)
Gene Symbol
BEST3
Additional Bestrophin 3 Products
Product Documents for Bestrophin 3 Antibody (OTI2H3)
Product Specific Notices for Bestrophin 3 Antibody (OTI2H3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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