beta B1 Crystallin Antibody (3D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00001414-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta B1 Crystallin Antibodies »

Product Specifications

Immunogen

CRYBB1 (NP_001878, 37 a.a. ~ 137 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TTLAPTTVPITSAKAAELPPGNYRLVVFELENFQGRRAEFSGECSNLADRGFDRVRSIIVSAGPWVAFEQSNFRGEMFILEKGEYPRWNTWSSSYRSDRLM

Specificity

CRYBB1 - crystallin, beta B1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta B1 Crystallin Antibody (3D9)

Western Blot: beta B1 Crystallin Antibody (3D9) [H00001414-M03]

Western Blot: beta B1 Crystallin Antibody (3D9) [H00001414-M03]

Western Blot: beta B1 Crystallin Antibody (3D9) [H00001414-M03] - Analysis of CRYBB1 expression in MCF-7 (Cat # L046V1).
ELISA: beta B1 Crystallin Antibody (3D9) [H00001414-M03]

ELISA: beta B1 Crystallin Antibody (3D9) [H00001414-M03]

ELISA: beta B1 Crystallin Antibody (3D9) [H00001414-M03] - Detection limit for recombinant GST tagged CRYBB1 is approximately 3ng/ml as a capture antibody.

Applications for beta B1 Crystallin Antibody (3D9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta B1 Crystallin

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3.

Alternate Names

Beta-B1 crystallin, beta-crystallin B1, CATCN3, crystallin, beta B1, eye lens structural protein

Entrez Gene IDs

1414 (Human)

Gene Symbol

CRYBB1

OMIM

600929 (Human)

UniProt

NP_001878 (Human)

Additional beta B1 Crystallin Products

Product Documents for beta B1 Crystallin Antibody (3D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta B1 Crystallin Antibody (3D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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