Beta Dystroglycan [p Tyr892] Antibody (M117)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23288

Novus Biologicals, part of Bio-Techne
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NBP3-23288
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M117

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Beta Dystroglycan Antibodies »

Product Summary for Beta Dystroglycan [p Tyr892] Antibody (M117)

Immunogen

Clone (M117) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 892 of human Beta Dystroglycan. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse Beta Dystroglycan. [UniProt# Q14118]

Modification

p Tyr892

Specificity

The antibody detects a 43 kDa protein on SDS-PAGE immunoblots of human HepG2 or HeLa cells treated with pervanadate, but not in control cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Beta Dystroglycan [p Tyr892] Antibody (M117)

Beta Dystroglycan [p Tyr892] Antibody (M117)

Western Blot: Beta Dystroglycan [p Tyr892] Antibody (M117) [NBP3-23288] -

Western Blot: Beta Dystroglycan [p Tyr892] Antibody (M117) [NBP3-23288] - Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-Beta Dystroglycan (lanes 1 & 2) and anti-Beta Dystroglycan (Tyr-892) (lanes 3 & 4).

Applications for Beta Dystroglycan [p Tyr892] Antibody (M117)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Beta Dystroglycan

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Gene Symbol

DAG1

Additional Beta Dystroglycan Products

Product Documents for Beta Dystroglycan [p Tyr892] Antibody (M117)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Dystroglycan [p Tyr892] Antibody (M117)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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