Beta Dystroglycan Antibody (S05-5B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19985

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19985-100ul
NBP3-19985-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-5B5

Concentration

0.3 mg/ml

View all Beta Dystroglycan Antibodies »

Product Summary for Beta Dystroglycan Antibody (S05-5B5)

Immunogen

A synthetic peptide of human Beta Dystroglycan (Uniprot # Q14118)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Beta Dystroglycan Antibody (S05-5B5)

Western Blot: Beta Dystroglycan Antibody (S05-5B5) [NBP3-19985]

Western Blot: Beta Dystroglycan Antibody (S05-5B5) [NBP3-19985]

Western Blot: Beta Dystroglycan Antibody (S05-5B5) [NBP3-19985] - Western blot detection of Beta Dystroglycan in MCF7, HepG2, Lncap cell lysates using NBP3-19985 (1:1000 diluted). Predicted band size: 27kDa. Observed band size: 42-44kDa.

Applications for Beta Dystroglycan Antibody (S05-5B5)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Beta Dystroglycan

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Gene Symbol

DAG1

Additional Beta Dystroglycan Products

Product Documents for Beta Dystroglycan Antibody (S05-5B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Dystroglycan Antibody (S05-5B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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