beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45731

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Canine, Monkey

Cited:

Human, Mouse, Non-species specific

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1C9

Concentration

1 mg/ml

View all beta-Galactosidase-1/GLB1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GLB1(NP_001073279) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

72.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1 .
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Applications for beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Galactosidase-1/GLB1

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Alternate Names

betaGalactosidase1, EBP, ELNR1, GLB1, Lactase

Gene Symbol

GLB1

Additional beta-Galactosidase-1/GLB1 Products

Product Documents for beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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