beta II Tubulin A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15581
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for beta II Tubulin A Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta II Tubulin A Antibody
Western Blot: beta II Tubulin A Antibody [NBP2-15581]
Western Blot: beta II Tubulin A Antibody [NBP2-15581] - beta II Tubulin A Antibody detects beta II Tubulin A protein by western blot analysis. A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract. D. 30 ug Raw 264.7 whole cell lysate/extract. E. 30 ug C2Cl2 whole cell lysate/extract. 10 % SDS-PAGE. beta II Tubulin A Antibody dilution: 1:2000Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody [NBP2-15581]
Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody [NBP2-15581] - HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: TUBB2A protein stained by TUBB2A antibody diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581]
Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581] - Mouse duodenum. beta Tubulin 2 antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for beta II Tubulin A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: beta II Tubulin A
Long Name
Tubulin beta-2A chain
Alternate Names
TUBB2, TUBB2A
Gene Symbol
TUBB2A
UniProt
Additional beta II Tubulin A Products
Product Documents for beta II Tubulin A Antibody
Product Specific Notices for beta II Tubulin A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.