beta Tubulin Antibody (D66)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1514
![Western Blot: beta Tubulin Antibody (D66) [NB600-1514]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0002.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # D66
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Sea urchin (Lytechinus pictus) sperm axonemal proteins.
Epitope
The epitope recognized by the antibody is localized in the C-terminal region of beta tubulin (amino acids 427-432 in sea urchin beta 2 tubulin), but is found in all isoforms of beta tubulin.
Reactivity Notes
Cross-reacts with Human, Monkey Mouse, Rat, Cow, Chicken, Chlamydomonas reinhardtii, Dog, Rabbit, Dinoflagellate and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic; Major constituent of microtubules.
Specificity
NB600-1514 recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta Tubulin.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
~50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for beta Tubulin Antibody (D66)
![Western Blot: beta Tubulin Antibody (D66) [NB600-1514]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0004.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514]")
Western Blot: beta Tubulin Antibody (D66) [NB600-1514]
Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-D66-Immunocytochemistry-Immunofluorescence-NB600-1514-img0003.jpg "Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514]")
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514]
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 0.5 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.![Western Blot: beta Tubulin Antibody (D66) [NB600-1514]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0001.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514]")
Western Blot: beta Tubulin Antibody (D66) [NB600-1514]
Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - beta Tubulin antibody Total cell extract of PC-12 was separated on SDS-PAGE. The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 ug/mL antibody 2. 1 ug/mL antibodyApplications for beta Tubulin Antibody (D66)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
0.5-1 ug/ml
Application Notes
ICC:(3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. Predicted molecular weight: 50 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody (D66)
Product Specific Notices for beta Tubulin Antibody (D66)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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