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beta Tubulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54728

Novus Biologicals, part of Bio-Techne
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NBP2-54728

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: GTGSGMGTLLISKIREEYPDRIINTFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta Tubulin Antibody

Western Blot: beta Tubulin Antibody [NBP2-54728]

Western Blot: beta Tubulin Antibody [NBP2-54728]

Western Blot: beta Tubulin Antibody [NBP2-54728] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-54728]

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-54728]

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-54728] - Staining of human lymph node shows cytoplasmic positivity in germinal center.
Western Blot: beta Tubulin Antibody [NBP2-54728]

Western Blot: beta Tubulin Antibody [NBP2-54728]

Western Blot: beta Tubulin Antibody [NBP2-54728] - Analysis in human cell line U-138MG.

Applications for beta Tubulin Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta Tubulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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