BHMT Antibody (3D6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04264

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D6

Format

BSA Free

Concentration

1.0 mg/ml

View all BHMT Antibodies »

Product Summary for BHMT Antibody (3D6) - BSA Free

Immunogen

Recombinant human BHMT (1-406aa) purified from E. coli

Specificity

NP_001704

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for BHMT Antibody (3D6) - BSA Free

Western Blot: BHMT Antibody (3D6)BSA Free [NBP1-04264]

Western Blot: BHMT Antibody (3D6)BSA Free [NBP1-04264]

Western Blot: BHMT Antibody (3D6) [NBP1-04264] - The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for BHMT Antibody (3D6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BHMT

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Alternate Names

betaine homocysteine methyltransferase, betaine-homocysteine methyltransferase, betaine--homocysteine S-methyltransferase, betaine--homocysteine S-methyltransferase 1, BHMT1, EC 2.1.1, EC 2.1.1.5

Gene Symbol

BHMT

Additional BHMT Products

Product Documents for BHMT Antibody (3D6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BHMT Antibody (3D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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