BID [p Ser78] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-260

Novus Biologicals, part of Bio-Techne
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NB500-260
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all BID Antibodies »

Product Summary for BID [p Ser78] Antibody

Immunogen

The immunogen recognized by this antibody maps to phosphorylated Serine 78 and surrounding residues mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122).

Modification

p Ser78

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BID [p Ser78] Antibody

Western Blot: BID [p Ser78] Antibody [NB500-260]

Western Blot: BID [p Ser78] Antibody [NB500-260]

Western Blot: BID [p Ser78] Antibody [NB500-260] - Detection of Mouse Phospho-BID (S78) after Treatment with EPE. Whole cell lysate (50 and 80 ug for WB; 1 mg for IP, 20% of IP loaded) from NIH3T3 cells that were treated with etoposide (100 mcM; 3 hours) or mock treated. Antibody used at 1 ug/ml.

Applications for BID [p Ser78] Antibody

Application
Recommended Usage

Western Blot

1:250-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BID

Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Long Name

BH3 Interacting Domain Death Agonist

Alternate Names

apoptic death agonist, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p22 BID, tbid

Entrez Gene IDs

12122 (Mouse)

Gene Symbol

BID

UniProt

P70444

Additional BID Products

Product Documents for BID [p Ser78] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BID [p Ser78] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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