BIG3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90759
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLKLLKNQEADQHSARLFIQSLEGLLPRLLSLSNVEEVDTALQNFASTFCSGMMHSPGFDGNSSLSFQMLMNADSLYTAAHCALLLNLKLSHG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BIG3 Antibody
![Western Blot: BIG3 Antibody [NBP1-90759]](https://resources.bio-techne.com/images/products/BIG3-Antibody-Western-Blot-NBP1-90759-img0007.jpg "Western Blot: BIG3 Antibody [NBP1-90759]")
Western Blot: BIG3 Antibody [NBP1-90759]
Western Blot: BIG3 Antibody [NBP1-90759] - Analysis in human cell line SK-BR-3.
Immunohistochemistry-Paraffin: BIG3 Antibody [NBP1-90759] -
Immunohistochemistry-Paraffin: BIG3 Antibody [NBP1-90759] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: BIG3 Antibody [NBP1-90759] -
Immunohistochemistry-Paraffin: BIG3 Antibody [NBP1-90759] -Staining of human prostate shows moderate cytoplasmic/membranous positivity in glandular cells and smooth muscle cells.Applications for BIG3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BIG3
Alternate Names
A7322, brefeldin A-inhibited guanine nucleotide-exchange protein 3, KIAA1244
Gene Symbol
KIAA1244
Additional BIG3 Products
Product Documents for BIG3 Antibody
Product Specific Notices for BIG3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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