Bilirubin Oxidase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44240
![Western Blot: Bilirubin Oxidase Antibody [NBP2-44240]](https://resources.bio-techne.com/images/products/Bilirubin-Oxidase-Antibody-Western-Blot-NBP2-44240-img0001.jpg "Western Blot: Bilirubin Oxidase Antibody [NBP2-44240]")
Key Product Details
Species Reactivity
Fungi
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Bilirubin Oxidase [Myrothecium verrucaria] (Uniprot: Q12737)
Reactivity Notes
Cross reactivity against Bilirubin Oxidase from other sources is unknown.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Bilirubin Oxidase Antibody
Western Blot: Bilirubin Oxidase Antibody [NBP2-44240]
Western Blot: Bilirubin Oxidase Antibody [NBP2-44240] - Analysis under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of
Bilirubin Oxidase Antibody
Goat anti Bilirubin Oxidase antibody ( lot 6533) was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 488 conjugated Donkey anti goat ( lot 21094 1:10K in TBS/ 1 hr RT) and Dylight 649 conjugated Donkey anti goat ( lot 20834 1:10K in TBS/) 1 hr RT. Images were collected using the BioRad VersaDoc SystemApplications for Bilirubin Oxidase Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Bilirubin Oxidase [Myrothecium verrucaria] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Bilirubin Oxidase
Alternate Names
Bilirubin oxidase, EC=1.3.3.5
UniProt
Additional Bilirubin Oxidase Products
Product Documents for Bilirubin Oxidase Antibody
Product Specific Notices for Bilirubin Oxidase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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