BIN1 Antibody (2F11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21675
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2F11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BIN1 Antibody (2F11) was produced in mouse by repeated immunizations with chimeric protein that encoded the human BIN1 BAR domain followed by hybridoma development. (Uniprot: O00499)
Reactivity Notes
Cross-reactivity with BIN1 from other sources has not been determined
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for BIN1 Antibody (2F11)
![Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675]](https://resources.bio-techne.com/images/products/BIN1-Antibody-2F11-Immunohistochemistry-Paraffin-NBP2-21675-img0001.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675]")
Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675]
Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.
BIN1 Antibody (2F11)
Immunohistochemistry of Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type (+/+) and null (-/-) mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.
BIN1 Antibody (2F11)
Western Blot of Mouse Antibody. Lane 1: Keratinocyte derived from Bin-1 wild type. Lane 2: Keratinocyte derived from Bin-1 null mice. Load: 35 ug per lane. Primary antibody: BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20 (PBS/T ) overnight at 4C. Bin1 isoforms (+exon13, -exon13). *indicates non-specific signal.Applications for BIN1 Antibody (2F11)
Application
Recommended Usage
ELISA
1:5000-1:50000
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
10-100 ul
Western Blot
1:500-1:1500
Application Notes
This product has been tested for use in Western Blot, ELISA, IP, and IHC. This antibody is suitable for. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: BIN1
Alternate Names
AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9
Gene Symbol
BIN1
UniProt
Additional BIN1 Products
Product Documents for BIN1 Antibody (2F11)
Product Specific Notices for BIN1 Antibody (2F11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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