BIN1 Antibody (99D)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21673
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 99D
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BIN1 Antibody (99D) was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development. (Uniprot: O00499)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for BIN1 Antibody (99D)
![Western Blot: BIN1 Antibody (99D) [NBP2-21673]](https://resources.bio-techne.com/images/products/BIN1-Antibody-99D-Western-Blot-NBP2-21673-img0001.jpg "Western Blot: BIN1 Antibody (99D) [NBP2-21673]")
Western Blot: BIN1 Antibody (99D) [NBP2-21673]
Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.
BIN1 Antibody (99D)
Flow Cytometry of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells. Stimulation: none. Primary antibody: Anti-IgD (control), Antibody (99D clone). Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.Applications for BIN1 Antibody (99D)
Application
Recommended Usage
ELISA
1:5000-1:50000
Flow Cytometry
0.5-1x10^6 cells
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
10-100 ul
Western Blot
1:500-1:1500
Application Notes
This product has been tested for use in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: BIN1
Alternate Names
AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9
Gene Symbol
BIN1
UniProt
Additional BIN1 Products
Product Documents for BIN1 Antibody (99D)
Product Specific Notices for BIN1 Antibody (99D)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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