BIN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89102

Novus Biologicals, part of Bio-Techne
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NBP1-89102
NBP1-89102-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BIN1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BIN1 Antibody

Western Blot: BIN1 Antibody [NBP1-89102]

Western Blot: BIN1 Antibody [NBP1-89102]

Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunocytochemistry/ Immunofluorescence: BIN1 Antibody [NBP1-89102]

Immunocytochemistry/ Immunofluorescence: BIN1 Antibody [NBP1-89102]

Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP1-89102] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102]

Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102]

Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining in human skeletal muscle and heart muscle tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.
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Applications for BIN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BIN1

BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Alternate Names

AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9

Gene Symbol

BIN1

Additional BIN1 Products

Product Documents for BIN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BIN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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