Biotin Antibody (rBTN/8819) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27043
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rBTN/8819
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Biotin Antibody (rBTN/8819) - Azide and BSA Free
Immunohistochemistry-Paraffin: Biotin Antibody (rBTN/8819) - Azide and BSA Free [NBP3-27043] -
Formalin-fixed, paraffin-embedded human kidney stained with Biotinylated Lambda Light Chain probe followed by Biotin Antibody (rBTN/8819) - Azide and BSA Free.Applications for Biotin Antibody (rBTN/8819) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml for 30 minutes at RT
Western Blot
0.5-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 0.5-1ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody (rBTN/8819) - Azide and BSA Free
Product Specific Notices for Biotin Antibody (rBTN/8819) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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