Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13901
![Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399) [NBP3-13901]](https://resources.bio-techne.com/images/products/Biotin-Vitamin-B7-or-Vitamin-H-Antibody-BTN399-Immunohistochemistry-Paraffin-NBP3-13901-img0001.jpg "Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399) [NBP3-13901]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BTN399
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14084)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399)
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399) [NBP3-13901]
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399) [NBP3-13901] - Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin (Vitamin B7 or Vitamin H) antibody (BTN399).Applications for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
Confocal Microscopy
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
In situ Nucleic Acid Hybridization
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
In situ Nucleic Acid Hybridization
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Biotin (Vitamin B7 or Vitamin H)
Alternate Names
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Additional Biotin (Vitamin B7 or Vitamin H) Products
Product Documents for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399)
Product Specific Notices for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN399)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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