Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BIRC6 Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from a sequence between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09 (GeneID 57448).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BIRC6 Antibody

Western Blot: BIRC6 Antibody [NB300-264]

Western Blot: BIRC6 Antibody [NB300-264]

Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264]

Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264]

Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).
Immunoprecipitation: BIRC6 Antibody [NB300-264]

Immunoprecipitation: BIRC6 Antibody [NB300-264]

Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for BIRC6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000-1:10000

Immunohistochemistry-Paraffin

1:2000-1:10000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:33815677) Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BIRC6

This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.

Long Name

Baculoviral IAP repeat-containing protein 6

Alternate Names

APOLLON, BRUCE, EC 2.3.2.27, KIAA1289

Entrez Gene IDs

57448 (Human)

Gene Symbol

BIRC6

UniProt

Q9NR09 (Human)

Additional BIRC6 Products

Product Documents for BIRC6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BIRC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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