Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all BIRC8 Antibodies »

Product Specifications

Immunogen

Antibody was raised with against a synthetic peptide corresponding to amino acids near the amino terminus of human ILP-2. The immunogen is located within the first 50 amino acids of ILP-2. Amino Acid Squence: TGYEARLITFGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BIRC8 Antibody - BSA Free

Western Blot: BIRC8 AntibodyBSA Free [NBP1-76669]

Western Blot: BIRC8 AntibodyBSA Free [NBP1-76669]

Western Blot: BIRC8 Antibody [NBP1-76669] - Human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 ug/ml.
BIRC8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BIRC8 Antibody - BSA Free [NBP1-76669] -

Immunocytochemistry/ Immunofluorescence: BIRC8 Antibody - BSA Free [NBP1-76669] - Immunocytochemistry of BIRC8 in HepG2 cells with BIRC8 antibody at 10 u/mL.
BIRC8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BIRC8 Antibody - BSA Free [NBP1-76669] -

Immunocytochemistry/ Immunofluorescence: BIRC8 Antibody - BSA Free [NBP1-76669] - Immunofluorescence of BIRC8 in HepG2 cells with BIRC8 antibody at 20 ug/mL.

Applications for BIRC8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1 ug/ml
Application Notes
A band at approximately 33 kDa can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BIRC8

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2. ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.

Long Name

Baculoviral IAP Repeat Containing 8

Alternate Names

ILP2, RNF136

Gene Symbol

BIRC8

UniProt

NP_203127 (Human)

Additional BIRC8 Products

Product Documents for BIRC8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BIRC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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