Blk Antibody (7A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00000640-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Blk Antibodies »

Product Specifications

Immunogen

BLK (AAH07371, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

BLK - B lymphoid tyrosine kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Blk Antibody (7A12)

Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02] - Analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M02), clone 7A12.Lane 1: BLK transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in PC-12.
Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02]

Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in Raw 264.7.
View 1 more images

Applications for Blk Antibody (7A12)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. FACS usage was reported in scientific literature.

Reviewed Applications

Read 1 review rated 4 using H00000640-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Blk

The gene encoding BLK is a member of the SRC family of protooncogenes and shows preferential expression in B-lymphoid cells. It is thought to function in a signal transductory pathway specific to this lineage.

Long Name

B Lymphoid Tyrosine Kinase

Alternate Names

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK nonreceptor tyrosine kinase, EC 2.7.10.2, MGC10442, MODY11, p55-Blk, tyrosine-protein kinase Blk

Entrez Gene IDs

640 (Human)

Gene Symbol

BLK

OMIM

191305 (Human)

UniProt

AAH07371 (Human)

Additional Blk Products

Product Documents for Blk Antibody (7A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Blk Antibody (7A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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