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Blooms Syndrome Protein Blm Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1590

Novus Biologicals, part of Bio-Techne
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NB100-1590

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for Blooms Syndrome Protein Blm Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1375 and the C-terminus (residue 1416) of mouse Bloom Syndrome Homolog using the numbering given in entry NP_031576.2 (GeneID 12144).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Blooms Syndrome Protein Blm Antibody

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Detection of Human and Mouse BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-1590 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Whole cell lysate from mouse NIH3T3 cells. BLM was also immunoprecipitated using rabbit anti-BLM antibodies NB100-1588 and NB100-1589.

Applications for Blooms Syndrome Protein Blm Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Blooms Syndrome Protein Blm

The Bloom's syndrome gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DexH-box-containing DNA and RNA helicases.

Long Name

Bloom syndrome protein

Alternate Names

BLM, EC 3.6.4.12, RecQ protein-like 3, RecQ2, RECQL3

Entrez Gene IDs

641 (Human); 12144 (Mouse)

Gene Symbol

BLM

UniProt

Additional Blooms Syndrome Protein Blm Products

Product Documents for Blooms Syndrome Protein Blm Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Blooms Syndrome Protein Blm Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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