Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all Blooms Syndrome Protein Blm Antibodies »

Product Summary for Blooms Syndrome Protein Blm Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Blooms Syndrome Protein Blm Antibody

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-BLM antibody NB100-324 (lot NB100-324-2) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot NB100-324-1) and rabbit anti-BLM antibody NB100-214. For blotting immunoprecipitated BLM, NB100-324 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Blooms Syndrome Protein Blm Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:10000 - 1:25000
Application Notes
NB 100-324 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * WB - 1:1,000-1:10,000 (ECL) IP - 1 to 4 ul/mg of lysate IHC / ICC - Not Determined *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Blooms Syndrome Protein Blm

The Bloom's syndrome gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DexH-box-containing DNA and RNA helicases.

Long Name

Bloom syndrome protein

Alternate Names

BLM, EC 3.6.4.12, RecQ protein-like 3, RecQ2, RECQL3

Entrez Gene IDs

641 (Human)

Gene Symbol

BLM

UniProt

NP_000048.1 (Human)

Additional Blooms Syndrome Protein Blm Products

Product Documents for Blooms Syndrome Protein Blm Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Blooms Syndrome Protein Blm Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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