Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BMAL1 Antibodies »

Product Summary for BMAL1 Antibody (OTI1C11) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human ARNTL(NP_001169) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMAL1 Antibody (OTI1C11) - Azide and BSA Free

Western Blot: BMAL1 Antibody (OTI1C11)Azide and BSA Free [NBP2-70207]

Western Blot: BMAL1 Antibody (OTI1C11)Azide and BSA Free [NBP2-70207]

Western Blot: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.
Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207]

Immunocytochemistry/ Immunofluorescence: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207]

Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL .
Immunohistochemistry: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207]

Immunohistochemistry: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207]

Immunohistochemistry: BMAL1 Antibody (OTI1C11) - Azide and BSA Free [NBP2-70207] - Staining of paraffin-embedded pancreas tissue using anti-BMAL1 mouse monoclonal antibody.
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Applications for BMAL1 Antibody (OTI1C11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BMAL1

Aryl hydrocarbon receptor nuclear translocator-like (ARNTL), also known as Bmal1 or Mop3 is a major component of the circadian clock oscillator. Specifically, BMAL-1 acts as a general dimerization partner for a subset of the bHLH-PAS (basic-helix-loop-helix-PER-ARNT-SIM) superfamily of transcription regulators, is involved in circadian rhythm generation. BMAL 1 interacts with MOP4, CLOCK, Hypoxia-inducible factor-1 alpha and -2 alpha. This interaction appears to be distinct from that of the more characterized general partner, ARNT (HIF-1Beta).

BMAL1 is highly expressed in the adult brain, skeletal muscle and heart, and has been associated with hypertension and type 2 diabetes. Because of its role in the circadian clock oscillator, BMAL-1 is also thought to be invloven seasonal affective disorder. Therefore, BMAL1 antibodies are useful for circadian studies as well as heart and brain research.

Long Name

Brain and Muscle ARNT-Like 1

Alternate Names

ARNTL, BHLHE5, BMAL1c, JAP3, MOP3, PASD3, TIC

Gene Symbol

BMAL1

Additional BMAL1 Products

Product Documents for BMAL1 Antibody (OTI1C11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BMAL1 Antibody (OTI1C11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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