BNIP1 Antibody (10K6T9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16403
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10K6T9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for BNIP1 Antibody (10K6T9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BNIP1 (Q12981). KFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BNIP1 Antibody (10K6T9)
![Western Blot: BNIP1 Antibody (10K6T9) [NBP3-16403]](https://resources.bio-techne.com/images/products/BNIP1-Antibody-10K6T9-Western-Blot-NBP3-16403-img0003.jpg "Western Blot: BNIP1 Antibody (10K6T9) [NBP3-16403]")
Western Blot: BNIP1 Antibody (10K6T9) [NBP3-16403]
Western Blot: BNIP1 Antibody (10K6T9) [NBP3-16403] - Western blot analysis of extracts of various cell lines, using BNIP1 Rabbit mAb (NBP3-16403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]](https://resources.bio-techne.com/images/products/BNIP1-Antibody-10K6T9-Immunohistochemistry-Paraffin-NBP3-16403-img0002.jpg "Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]")
Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]
Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403] - Immunohistochemistry of paraffin-embedded mouse spinal cord using BNIP1 Rabbit mAb (NBP3-16403) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]](https://resources.bio-techne.com/images/products/BNIP1-Antibody-10K6T9-Immunohistochemistry-Paraffin-NBP3-16403-img0001.jpg "Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]")
Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403]
Immunohistochemistry-Paraffin: BNIP1 Antibody (10K6T9) [NBP3-16403] - Immunohistochemistry of paraffin-embedded rat brain using BNIP1 Rabbit mAb (NBP3-16403) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for BNIP1 Antibody (10K6T9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BNIP1
Alternate Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, BCL2/adenovirus E1B 19kDa interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1, Nip1, SEC20, SEC20L, Transformation-related gene 8 protein, TRG-8, vesicle transport protein SEC20
Gene Symbol
BNIP1
Additional BNIP1 Products
Product Documents for BNIP1 Antibody (10K6T9)
Product Specific Notices for BNIP1 Antibody (10K6T9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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