BNIP1 Antibody (OTI2B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70257

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BNIP1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-199 of human BNIP1 (NP_001196) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BNIP1 Antibody (OTI2B3) - Azide and BSA Free

Western Blot: BNIP1 Antibody (OTI2B3)Azide and BSA Free [NBP2-70257]

Western Blot: BNIP1 Antibody (OTI2B3)Azide and BSA Free [NBP2-70257]

Western Blot: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1.
Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257]

Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257]

Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257]

Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257]

Immunohistochemistry: BNIP1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-70257] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for BNIP1 Antibody (OTI2B3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BNIP1

BNIP1 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Alternate Names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, BCL2/adenovirus E1B 19kDa interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1, Nip1, SEC20, SEC20L, Transformation-related gene 8 protein, TRG-8, vesicle transport protein SEC20

Gene Symbol

BNIP1

Additional BNIP1 Products

Product Documents for BNIP1 Antibody (OTI2B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BNIP1 Antibody (OTI2B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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