BNIP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15602
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human BNIP2. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (86%), Xenopus tropicalis (86%).
Localization
Cytoplasm; perinuclear region
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BNIP2 Antibody
![Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP2-15602]](https://resources.bio-techne.com/images/products/BNIP2-Antibody-Immunohistochemistry-Paraffin-NBP2-15602-img0002.jpg "Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP2-15602]")
Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP2-15602]
Immunohistochemistry-Paraffin: BNIP2 Antibody [NBP2-15602] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: BNIP2 Antibody [NBP2-15602] -
Immunocytochemistry/ Immunofluorescence: BNIP2 Antibody [NBP2-15602] - BNIP2 antibody detects BNIP2 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BNIP2 protein stained by BNIP2 antibody (NBP2-15602) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI .
Western Blot: BNIP2 Antibody [NBP2-15602] -
Western Blot: BNIP2 Antibody [NBP2-15602] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody (NBP2-15602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for BNIP2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BNIP2
Alternate Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2, BCL2/adenovirus E1B 19kDa interacting protein 2, BCL2/adenovirus E1B 19kD-interacting protein 2, BNIP-2, NIP2
Gene Symbol
BNIP2
UniProt
Additional BNIP2 Products
Product Specific Notices for BNIP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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