BNIP3 Antibody (6A5F7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61715

Novus Biologicals, part of Bio-Techne
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NBP2-61715
NBP2-61715-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 6A5F7

Format

BSA Free

Concentration

1 mg/ml

View all BNIP3 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BNIP3 (AA: 50-155) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BNIP3 Antibody (6A5F7) - BSA Free

Western Blot: BNIP3 Antibody (6A5F7)BSA Free [NBP2-61715]

Western Blot: BNIP3 Antibody (6A5F7)BSA Free [NBP2-61715]

Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)
Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody (6A5F7) - BSA Free [NBP2-61715]

Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody (6A5F7) - BSA Free [NBP2-61715]

Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) - BSA Free [NBP2-61715]

Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) - BSA Free [NBP2-61715]

Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of rectum cancer tissues using BNIP3 mouse mAb with DAB staining.
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Applications for BNIP3 Antibody (6A5F7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50 - 1:250

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BNIP3

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.;

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

Nip3

Gene Symbol

BNIP3

Additional BNIP3 Products

Product Documents for BNIP3 Antibody (6A5F7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BNIP3 Antibody (6A5F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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