BOB1 Antibody (4A5)

Detection of BOB1/POU2AF1 in Human Tonsil via seqIF™ staining on COMET™

BOB1/POU2AF1 Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human , Monoclonal Antibody (Catalog # NBP3-26373) at 1:100 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] -

Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] -

Immunohistochemistry: BOB1 Antibody (4A5) [NBP3-26373] - Image of BOB1 Antibody (4A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.