BOB1 Antibody (BOB1/7468)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20301
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # BOB1/7468
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary)
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Description
Positive Controls: Raji or Ramos cells. Spleen or tonsil (IHC).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for BOB1 Antibody (BOB1/7468)
BOB1 Antibody (BOB1/7468)
Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 antibody (BOB1/7468). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
BOB1 Antibody (BOB1/7468)
IHC analysis of formalin-fixed, paraffin-embedded human placenta. Negative tissue control using BOB1 antibody (BOB1/7468) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
BOB1 Antibody (BOB1/7468)
Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 antibody (BOB1/7468). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for BOB1 Antibody (BOB1/7468)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BOB1
Alternate Names
B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1
Gene Symbol
POU2AF1
Additional BOB1 Products
Product Documents for BOB1 Antibody (BOB1/7468)
Product Specific Notices for BOB1 Antibody (BOB1/7468)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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