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BOB1 Antibody (BOB1/7469) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20971

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # BOB1/7469

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Positive Controls: Raji or Ramos cells. Spleen or tonsil (IHC).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for BOB1 Antibody (BOB1/7469) - Azide and BSA Free

BOB1 Antibody (BOB1/7469) - Azide and BSA Free

BOB1 Antibody (BOB1/7469)

IHC analysis of formalin-fixed, paraffin-embedded human skin in tonsil. Negative tissue control using BOB1 antibody (BOB1/7469) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
BOB1 Antibody (BOB1/7469) - Azide and BSA Free

BOB1 Antibody (BOB1/7469)

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 antibody (BOB1/7469). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
BOB1 Antibody (BOB1/7469) - Azide and BSA Free

BOB1 Antibody (BOB1/7469)

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 antibody (BOB1/7469). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for BOB1 Antibody (BOB1/7469) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

Additional BOB1 Products

Product Documents for BOB1 Antibody (BOB1/7469) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BOB1 Antibody (BOB1/7469) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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