Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BOB1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POU2AF1 (NP_006226) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BOB1 Antibody (OTI1E11) - Azide and BSA Free

Western Blot: BOB1 Antibody (OTI1E11)Azide and BSA Free [NBP2-71223]

Western Blot: BOB1 Antibody (OTI1E11)Azide and BSA Free [NBP2-71223]

Western Blot: BOB1 Antibody (OTI1E11) - Azide and BSA Free [NBP2-71223] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BOB1.
Western Blot: BOB1 Antibody (OTI1E11)Azide and BSA Free [NBP2-71223]

Western Blot: BOB1 Antibody (OTI1E11)Azide and BSA Free [NBP2-71223]

Western Blot: BOB1 Antibody (OTI1E11) - Azide and BSA Free [NBP2-71223] - Analysis of extracts (15ug) from 9 Human tissue. (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon: 9:Spleen)

Applications for BOB1 Antibody (OTI1E11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

Additional BOB1 Products

Product Documents for BOB1 Antibody (OTI1E11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BOB1 Antibody (OTI1E11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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