BOP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21389

Novus Biologicals, part of Bio-Techne
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NBP1-21389
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all BOP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human block of proliferation 1 using the numbering given in entry NP_056016.1 (GeneID 23246).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BOP1 Antibody

Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389]

Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389]

Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389] - FFPE section of human colon carcinoma (left) and mouse teratoma. Antibody:Affinity purified rabbit anti-BOP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: BOP1 Antibody [NBP1-21389]

Immunoprecipitation: BOP1 Antibody [NBP1-21389]

Immunoprecipitation: BOP1 Antibody [NBP1-21389] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-21388 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Applications for BOP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BOP1

BOP1 (block of proliferation 1) is a component of the PeBoW (Pes1, Bop, WDR12) complex that plays a role in the processing of 28S and 5.8S ribosomal RNAs and the formation of the 60S ribosome. BOP1 contains seven WD repeats and was originally identified as a cDNA which inhibited cell proliferation. The deregulation of the BOP1 gene is proposed to contribute to colorectal tumorigenesis.

Alternate Names

block of proliferation 1, KIAA0124Block of proliferation 1 protein, ribosome biogenesis protein BOP1

Entrez Gene IDs

23246 (Human)

Gene Symbol

BOP1

UniProt

NP_056016.1 (Human)

Additional BOP1 Products

Product Documents for BOP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BOP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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