Borealin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22832

Novus Biologicals, part of Bio-Techne
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NBP1-22832
NBP1-22832SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Borealin Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human Borealin (within residues 200-250). [Swiss-Prot# Q53HL2]

Reactivity Notes

Immunogen sequence has 92% identity with mouse and rat proteins.

Localization

Nucleus, nucleolus. Cytoplasm. Centromere. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Borealin Antibody

Western Blot: Borealin Antibody [NBP1-22832]

Western Blot: Borealin Antibody [NBP1-22832]

Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.

Applications for Borealin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

2 ug/ml
Application Notes
This Borealin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Borealin

Encoded by gene CDCA8, borealin forms an important component of mitosis regulating chromosomal passenger complex (CPC complex - BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which is known for ensuring correct chromosome alignment/segregation and is required for chromatin-induced microtubule stabilization as well as spindle assembly. Borealin governs CPC to centromeric DNA and is a major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Besides associating with CPC complex subunits, borealin interact with SENP3, UBE2I and RANBP2 which are involved its sumoylation. Borealin is expressed in a cell cycle-dependent manner and the levels of endogenous borealin significantly increase during mitosis. TTK can phosphorylated borealin at Thr-88, Thr94, Thr-169 and Thr-230 and and mutation of S219 to alanine in borealin causes defects in mitotic progression advocating for the importance of phosphorylation in borealin function. Mutation of additional sites creates a form of borealin that is defective in localizing to the centromere, binding to Sgo1 and Sgo2, and in coordinating chromosome alignment.

Alternate Names

BOR, borealin, cell division cycle associated 8, Cell division cycle-associated protein 8, Dasra B, DasraB, Dasra-B, FLJ10468, FLJ12042, hDasra-B, MESRGP, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein

Entrez Gene IDs

55143 (Human)

Gene Symbol

CDCA8

UniProt

Q53HL2 (Human)

Additional Borealin Products

Product Documents for Borealin Antibody

Product Datasheets

Certificate of Analysis

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Download SDS

Product Specific Notices for Borealin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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