BORIS Antibody (20B11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52405

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 20B11

Format

BSA Free

Concentration

1 mg/ml

View all BORIS Antibodies »

Product Specifications

Immunogen

Synthetic peptide within the BORIS C-terminal domain (aa 614-648) (CG)GEMFPVACRETTARVKEE (NB - the first two aa do not belong to BORIS)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BORIS Antibody (20B11) - BSA Free

Western Blot: BORIS Antibody (20B11)BSA Free [NBP2-52405]

Western Blot: BORIS Antibody (20B11)BSA Free [NBP2-52405]

Western Blot: BORIS Antibody (20B11) [NBP2-52405] - Antibody from clone 20B11 specifically recognises the C terminal domain of BORIS, and the endogenous and exogenous BORIS protein. Cell lysates were resolved by SDS-PAGE, blotted and probed with the original mouse supernatant of the 20B11 clone. Positions of BORIS and the C-terminal domain of BORIS are indicated. B, bacterially expressed BORIS; 293T cells transfected with 0.5ug of the plasmid expressing BORIS; M, Molecular marker.
Immunohistochemistry: BORIS Antibody (20B11) - BSA Free [NBP2-52405]

Immunohistochemistry: BORIS Antibody (20B11) - BSA Free [NBP2-52405]

Immunohistochemistry: BORIS Antibody (20B11) [NBP2-52405] - Analysis using the supernatants from the clone 20B11 at following dilutions: (A) 20B11 undiluted supernatant and (B) 20B11 at 1:25. (B) Cells have been counterstained with the nuclear marker hematoxylin (blue). Images were taken at x60 magnification using the Olympus BX41 microscope. Expression of BORIS protein is indicated by the presence of brown staining, with a pattern characteristic for BORIS ( both, cytoplasmic and nuclear).
Immunoprecipitation: BORIS Antibody (20B11) - BSA Free [NBP2-52405]

Immunoprecipitation: BORIS Antibody (20B11) - BSA Free [NBP2-52405]

Immunoprecipitation: BORIS Antibody (20B11) [NBP2-52405] - The 83-kDa protein can be immunoprecipitated with the mouse anti- BORIS C terminal specific antibody. Proteins were immunoprecipitated from the MCF7 cell extracts with the mouse anti- BORIS C terminal antibody 20B11 in their original supernatant form using the following volumes: (1) 400 ul of 20B11; (2) 800 ul of 20B11; (3) Input; (4) MCF7 cell lysates as BORIS positive control. The extracts from MCF7 cells were incubated overnight at 40C followed by 2 hour incubation with protein G sepharose beads. Proteins were resolved by SDS-PAGE, and Western blot analysis was performed with the concentrated form of the mouse anti- BORIS N terminal (4A7) specific antibody (1:500).

Applications for BORIS Antibody (20B11) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): MCF7 cell lysates.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BORIS

BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.

Alternate Names

BORISHMGB1L1, BORIS-like protein, Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein)-like, CT27MGC163358, CTCF paralog, CTCF-like protein, CTCF-T, dJ579F20.2, HMG-1L1, MGC169105, MGC169106, putative high mobility group protein 1-like 1, putative high mobility group protein B1-like 1, transcriptional repressor CTCFL, Zinc finger protein CTCF-T

Gene Symbol

CTCFL

Additional BORIS Products

Product Documents for BORIS Antibody (20B11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BORIS Antibody (20B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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