Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BORIS Antibodies »

Product Specifications

Immunogen

This affinity purified BORIS Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein. (Uniprot: Q8NI51)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with BORIS proteins from human and chimpanzee. Partial reactivity may occur against BORIS from mouse, dog and rat sources based on varying degrees of homology to the immunizing sequence. Reactivity against homologues from other sources is not known.

Localization

Nuclear and cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity-purified antibody is directed against the human BORIS protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for BORIS Antibody

Western Blot: BORIS Antibody [NB100-2028]

Western Blot: BORIS Antibody [NB100-2028]

Western Blot: BORIS Antibody [NB100-2028] - Analysis of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at 75 kDa is observed.
BORIS Antibody

BORIS Antibody

Western blot using Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at ~75 kDa is observed. Molecular weight estimation was made by comparison to prestained MW markers as indicated.

Applications for BORIS Antibody

Application
Recommended Usage

ELISA

1:2000-1:10000

Western Blot

1:200-1:2000
Application Notes
This product has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BORIS

BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.

Alternate Names

BORISHMGB1L1, BORIS-like protein, Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein)-like, CT27MGC163358, CTCF paralog, CTCF-like protein, CTCF-T, dJ579F20.2, HMG-1L1, MGC169105, MGC169106, putative high mobility group protein 1-like 1, putative high mobility group protein B1-like 1, transcriptional repressor CTCFL, Zinc finger protein CTCF-T

Entrez Gene IDs

140690 (Human)

Gene Symbol

CTCFL

UniProt

Q8NI51 (Human)

Additional BORIS Products

Product Documents for BORIS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BORIS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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