BPGM Antibody (2E11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59493

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E11

Format

BSA Free

Concentration

1 mg/ml

View all BPGM Antibodies »

Product Specifications

Immunogen

Recombinant human BPGM (1-259aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for BPGM Antibody (2E11) - BSA Free

Western Blot: BPGM Antibody (2E11)BSA Free [NBP2-59493]

Western Blot: BPGM Antibody (2E11)BSA Free [NBP2-59493]

Western Blot: BPGM Antibody (2E11) [NBP2-59493] - Lane 1: 293T cell lysates, Lane 2: BPGM transfected 293T cell lysates
Immunocytochemistry/ Immunofluorescence: BPGM Antibody (2E11) - BSA Free [NBP2-59493]

Immunocytochemistry/ Immunofluorescence: BPGM Antibody (2E11) - BSA Free [NBP2-59493]

Immunocytochemistry/Immunofluorescence: BPGM Antibody (2E11) [NBP2-59493] - Analysis of BPGM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BPGM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: BPGM Antibody (2E11) - BSA Free [NBP2-59493]

Flow Cytometry: BPGM Antibody (2E11) - BSA Free [NBP2-59493]

Flow Cytometry: BPGM Antibody (2E11) [NBP2-59493] - Analysis of BPGM in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

Applications for BPGM Antibody (2E11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BPGM

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds toand decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPGsynthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also hasphosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen.Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein,have been identified. (provided by RefSeq)

Alternate Names

2,3-bisphosphoglycerate mutase, bisphosphoglycerate mutase, BPG-dependent PGAM, EC 3.1.3.132,3-bisphosphoglycerate synthase, EC 5.4.2.1, EC 5.4.2.4, erythrocyte 2,3-bisphosphoglycerate mutase2,3-bisphosphoglycerate mutase, erythrocyte

Gene Symbol

BPGM

Additional BPGM Products

Product Documents for BPGM Antibody (2E11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BPGM Antibody (2E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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