BPGM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86377

Novus Biologicals, part of Bio-Techne
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NBP1-86377
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BPGM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BPGM Antibody

Western Blot: BPGM Antibody [NBP1-86377]

Western Blot: BPGM Antibody [NBP1-86377]

Western Blot: BPGM Antibody [NBP1-86377] - Analysis in control (vector only transfected HEK293T lysate) and BPGM over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: BPGM Antibody [NBP1-86377]

Immunocytochemistry/ Immunofluorescence: BPGM Antibody [NBP1-86377]

Immunocytochemistry/Immunofluorescence: BPGM Antibody [NBP1-86377] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86377]

Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86377]

Immunohistochemistry-Paraffin: BPGM Antibody [NBP1-86377] - Staining in human placenta and pancreas tissues using anti-BPGM antibody. Corresponding BPGM RNA-seq data are presented for the same tissues.
View 2 more images

Applications for BPGM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BPGM

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds toand decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPGsynthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also hasphosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen.Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein,have been identified. (provided by RefSeq)

Alternate Names

2,3-bisphosphoglycerate mutase, bisphosphoglycerate mutase, BPG-dependent PGAM, EC 3.1.3.132,3-bisphosphoglycerate synthase, EC 5.4.2.1, EC 5.4.2.4, erythrocyte 2,3-bisphosphoglycerate mutase2,3-bisphosphoglycerate mutase, erythrocyte

Gene Symbol

BPGM

Additional BPGM Products

Product Documents for BPGM Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BPGM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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