BRAF35 Antibody (1F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00010362-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRAF35 Antibodies »

Product Specifications

Immunogen

HMG20B (NP_006330, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNV

Reactivity Notes

Human. Other species not tested.

Specificity

HMG20B - high-mobility group 20B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for BRAF35 Antibody (1F6)

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01]

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01]

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - HMG20B monoclonal antibody (M01), clone IF6 Analysis of HMG20B expression in HeLa.
Immunocytochemistry/ Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01]

Immunocytochemistry/ Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01]

Immunocytochemistry/Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01]

Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01]

Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. Antibody concentration 1.8 ug/ml.
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Applications for BRAF35 Antibody (1F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for ELISA, immunofluoresence and immunohistochemistry (paraffin).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRAF35

BRAF35 is required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters

Alternate Names

BRAF35Sox-like transcriptional factor, high-mobility group 20B, HMG domain-containing protein 2, HMG domain-containing protein HMGX2, HMGX2FLJ26127, HMGXB2member 1-related, SMARCE1R, SMARCE1-related protein

Entrez Gene IDs

10362 (Human)

Gene Symbol

HMG20B

OMIM

605535 (Human)

UniProt

NP_006330 (Human)

Additional BRAF35 Products

Product Documents for BRAF35 Antibody (1F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BRAF35 Antibody (1F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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