BRAF35 Antibody (PCRP-HMG20B-1B5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20467
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PCRP-HMG20B-1B5
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Synthetic peptide (exact sequence is proprietary)
Localization
Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: Jurkat, A431 or HeLa cells. Ubiquitous expression in adult tissues.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for BRAF35 Antibody (PCRP-HMG20B-1B5)
Flow cytometric analysis of PFA-fixed HeLa cells. BRAF35 BRAF35 antibody (PCRP-HMG20B-1B5)) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
BRAF35 Antibody (PCRP-HMG20B-1B5)
Analysis of Protein Array containing more than 19,000 full-length human proteins using BRAF35 Mouse Monoclonal Antibody (PCRP-HMG20B-1B5).
BRAF35 Antibody (PCRP-HMG20B-1B5)
Formalin-fixed, paraffin-embedded human colon stained with BRAF35 antibody (PCRP-HMG20B-1B5). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for BRAF35 Antibody (PCRP-HMG20B-1B5)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug of protein or 1ml of lysate
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BRAF35
Alternate Names
BRAF35Sox-like transcriptional factor, high-mobility group 20B, HMG domain-containing protein 2, HMG domain-containing protein HMGX2, HMGX2FLJ26127, HMGXB2member 1-related, SMARCE1R, SMARCE1-related protein
Gene Symbol
HMG20B
Additional BRAF35 Products
Product Documents for BRAF35 Antibody (PCRP-HMG20B-1B5)
Product Specific Notices for BRAF35 Antibody (PCRP-HMG20B-1B5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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