Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRCA1 Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Breast Cancer Gene 1 (GeneID 672) around serine 1524 according to the numbering given in entry NP_009225.1.

Modification

p Ser1524

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRCA1 [p Ser1524] Antibody

Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200]

Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200]

Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200]

Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200]

Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Nuclear extract (50 ug) from HeLa cells mock irradiated or irradiated with 10 Gy of ionizing radiation. Antibody used at 0.5 ug/ml.
Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200]

Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200]

Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Applications for BRCA1 [p Ser1524] Antibody

Application
Recommended Usage

Immunoprecipitation

2.5 ug/mg of lysate

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCA1

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human)

Gene Symbol

BRCA1

UniProt

P38398 (Human)

Additional BRCA1 Products

Product Documents for BRCA1 [p Ser1524] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRCA1 [p Ser1524] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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