BRD4 [p Thr204] Antibody (3V5R1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16958
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3V5R1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for BRD4 [p Thr204] Antibody (3V5R1)
Immunogen
A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1). ASTPP
Modification
p Thr 204
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 [p Thr204] Antibody (3V5R1)
Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of various cell lines, using (NBP3-16958) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of NIH/3T3 cells, using (NBP3-16958) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Applications for BRD4 [p Thr204] Antibody (3V5R1)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Gene Symbol
BRD4
Additional BRD4 Products
Product Documents for BRD4 [p Thr204] Antibody (3V5R1)
Product Specific Notices for BRD4 [p Thr204] Antibody (3V5R1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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