Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRD8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 25 and 75 of human Bromodomain containing 8 using the numbering given in TrEMBL entry Q969M9 (GeneID 10902).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD8 Antibody

Western Blot: BRD8 Antibody [NB100-79780]

Western Blot: BRD8 Antibody [NB100-79780]

Western Blot: BRD8 Antibody [NB100-79780] - Detection of Human BRD8 by Western Blot. Samples: Nuclear extract (50 and 15 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: BRD8 Antibody [NB100-79780]

Immunoprecipitation: BRD8 Antibody [NB100-79780]

Immunoprecipitation: BRD8 Antibody [NB100-79780] - Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for IP at 6 ug per reaction. BRD8 was also immunoprecipitated by rabbit anti-BRD8 antibody NB100-79781. For blotting immunoprecipitated BRD8, NB100-79780 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BRD8 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRD8

The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced transcript variants that encode distinct isoforms have been identified.

Long Name

Bromodomain-containing protein 8

Alternate Names

SMAP, TrCP120

Entrez Gene IDs

10902 (Human)

Gene Symbol

BRD8

UniProt

Q969M9 (Human)

Additional BRD8 Products

Product Documents for BRD8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BRD8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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