Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRD8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 200 and 250 of human Bromodomain containing 8 using the numbering given in TrEMBL entry Q969M9 (GeneID 10902).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD8 Antibody

Western Blot: BRD8 Antibody [NB100-79781]

Western Blot: BRD8 Antibody [NB100-79781]

Western Blot: BRD8 Antibody [NB100-79781] - Detection of Human BRD8 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79781 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: BRD8 Antibody [NB100-79781]

Immunohistochemistry: BRD8 Antibody [NB100-79781]

Immunohistochemistry: BRD8 Antibody [NB100-79781] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BRD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Western Blot: BRD8 Antibody [NB100-79781]

Western Blot: BRD8 Antibody [NB100-79781]

Western Blot: BRD8 Antibody [NB100-79781] - A) Nuclear extract (NE; 5, 15 and 50 mcg) from HeLa cells. B) Nuclear extract (1 mg) from HeLa cells. Antibodies: A) Rabbit anti-BRD8 antibody, NB100-79781 used at the indicated concentrations for WB. B). Rabbit anti-BRD8 antibodies, NB100-79780, and NB100-79781 used at 1 and 5 mcg/mg lysate for IP. Immunoprecipitated BRD8 was detected using anti-BRD8 antibody NB100-79781 at 0.1 mcg/ml.
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Applications for BRD8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-8 ug/mg lysate

Western Blot

1:10000-1:25000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRD8

BRD8 may act as a coactivator during transcriptional activation by hormone activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.

Long Name

Bromodomain-containing protein 8

Alternate Names

SMAP, TrCP120

Entrez Gene IDs

10902 (Human)

Gene Symbol

BRD8

UniProt

Q969M9 (Human)

Additional BRD8 Products

Product Documents for BRD8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BRD8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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