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BRDT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40662

Novus Biologicals, part of Bio-Techne
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NB110-40662-100ug

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QSDIMTMFENNFD corresponding to C-Terminus according to NP_001717.2, NP_997072.1.

Specificity

Variants (NP_001717.2; NP_997072.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for BRDT Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human testis lysate after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of 108 kDa band according to NP_001717. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRDT

The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins.

Long Name

Bromodomain testis-specific protein

Alternate Names

CT9, RING3-like protein

Entrez Gene IDs

676 (Human); 114642 (Mouse)

Gene Symbol

BRDT

UniProt

Additional BRDT Products

Product Documents for BRDT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRDT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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