BRE Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32304
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for BRE Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human BRE. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRE Antibody
Knockout Validated: BRE Antibody [NBP1-32304]
Knockout Validated: BRE Antibody [NBP1-32304] - Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Immunocytochemistry/ Immunofluorescence: BRE Antibody [NBP1-32304]
Immunocytochemistry/Immunofluorescence: BRE Antibody [NBP1-32304] - analysis of HeLa, using BRE antibody at 1:200 dilution.Western Blot: BRE Antibody [NBP1-32304]
Western Blot: BRE Antibody [NBP1-32304] - Sample: 1000 ug 293T whole cell lysate/extract A. Control with 2. 5 ug of preimmune rabbit IgG, B. Immunoprecipitation of BRE protein by 2. 5 ug of BRE antibody 10% SDS-PAGE gel.Applications for BRE Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BRE
Alternate Names
brain and reproductive organ-expressed (TNFRSF1A modulator), BRCA1/BRCA2-containing complex subunit 45, subunit 4
Gene Symbol
BABAM2
Additional BRE Products
Product Documents for BRE Antibody
Product Specific Notices for BRE Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.