BRE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88359

Novus Biologicals, part of Bio-Techne
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NBP1-88359
NBP1-88359-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRE Antibodies »

Product Summary for BRE Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWDIIFNAQYPELPPDFIFGEDAEFLPDPSALQNLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRE Antibody

Western Blot: BRE Antibody [NBP1-88359]

Western Blot: BRE Antibody [NBP1-88359]

Western Blot: BRE Antibody [NBP1-88359] - Analysis in control (vector only transfected HEK293T lysate) and BRE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: BRE Antibody [NBP1-88359]

Immunocytochemistry/ Immunofluorescence: BRE Antibody [NBP1-88359]

Immunocytochemistry/Immunofluorescence: BRE Antibody [NBP1-88359] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: BRE Antibody [NBP1-88359]

Immunohistochemistry-Paraffin: BRE Antibody [NBP1-88359]

Immunohistochemistry-Paraffin: BRE Antibody [NBP1-88359] - Staining of human kidney shows strong positivity in tubules.

Applications for BRE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRE

BRE is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRC

Alternate Names

brain and reproductive organ-expressed (TNFRSF1A modulator), BRCA1/BRCA2-containing complex subunit 45, subunit 4

Gene Symbol

BABAM2

Additional BRE Products

Product Documents for BRE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BRE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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