BRF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1120

Novus Biologicals, part of Bio-Techne
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NB100-1120
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BRF2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QAARQAATSVPNPP corresponding to C-Terminus according to NP_060780.2.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BRF2 Antibody

Western Blot: BRF2 Antibody [NB100-1120]

Western Blot: BRF2 Antibody [NB100-1120]

Western Blot: BRF2 Antibody [NB100-1120] - (1.5ug/ml) Annlysis of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for BRF2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in Hela and human placenta lysates (predicted MW of 48 kDa band according to NP_060780).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRF2

BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.

Alternate Names

B-related factor 2, BRF-2, BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, BRFUhTFIIIB50, FLJ11052, hBRFU, TFIIIB50RNA polymerase III transcription initiation factor BRFU, transcription factor IIB- related factor, TFIIIB50, transcription factor IIIB 50 kDa subunit

Entrez Gene IDs

55290 (Human)

Gene Symbol

BRF2

UniProt

NP_060780. (Human)

Additional BRF2 Products

Product Documents for BRF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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