Brg1 Antibody (4N9C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15773

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15773-100ul
NBP3-15773-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4N9C4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Brg1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Brg1 (P51532). LQMAVQGKRPMPGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPKPWPEGPMANAAAPTSTPQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brg1 Antibody (4N9C4)

Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773]

Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773]

Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Western blot analysis of extracts of various cell lines, using Brg1 antibody (NBP3-15773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (4N9C4) [NBP3-15773]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (4N9C4) [NBP3-15773]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunofluorescence analysis of NIH-3T3 cells using Brg1 antibody (NBP3-15773). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773]

Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773]

Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] - Immunohistochemistry of paraffin-embedded mouse spleen using Brg1 antibody (NBP3-15773) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Brg1 Antibody (4N9C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (4N9C4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (4N9C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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