Brg1 Antibody (SN20-03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67720

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN20-03

Concentration

1 mg/ml

View all Brg1 Antibodies »

Product Summary for Brg1 Antibody (SN20-03)

Immunogen

Synthetic peptide within human Brg1 aa 240-280. (SwissProt: P51532 Human; SwissProt: Q3TKT4 Mouse; SwissProt: Q8K1P7 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brg1 Antibody (SN20-03)

Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720]

Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720]

Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720]

Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720]

Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for Brg1 Antibody (SN20-03)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:50-1:1000

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (SN20-03)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (SN20-03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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