Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Brg1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4. The immunogen is located within amino acids 1420 - 1470 of SMARCA4. Amino Acid Squence: SRDKDDESKKQKKRGRPPAE

Specificity

Multiple isoforms of SMARCA4 are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Brg1 Antibody - BSA Free

Western Blot: Brg1 AntibodyBSA Free [NBP2-41270]

Western Blot: Brg1 AntibodyBSA Free [NBP2-41270]

Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: Brg1 Antibody - BSA Free [NBP2-41270]

Immunohistochemistry-Paraffin: Brg1 Antibody - BSA Free [NBP2-41270]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 2 ug/ml.

Immunohistochemistry: Brg1 Antibody [NBP2-41270] -

Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 20 ug/ml.
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Applications for Brg1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Brg1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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